GC Image GCxGC-HRMS Edition Users' Guide
New Features and Improvements in Version 2.7
GC Image GCxGC-HRMS Edition 2.7 has many new features and
improvements. See the GC Image GCxGC-HRMS Edition
Users' Guide for full documentation.
What's New in Version 2.7 Release 2 (October 2017)
Improved Support for Heart-Cutting and Variable Modulation
GC Image GCxGC-HRMS Edition 2.7 Release 2 has improved support for small
chromatograms, such as ones acquired with heart-cutting including:
- Support zooming into tiny regions on 2D View.
- Allow arbitrary scales to be set through the tool bar.
- Allow more projection lines to be used by 1D View.
- Allow creating an edge-to-edge graphical area (rectangle,
polygon, and polyline) for manual integration on chromatograms of any size.
For chromatograms acquired with variable modulation, the software has the following improvements:
- Image Attributes now displays a Variable Modulation table if available.
- Column I dimensions of template graphics are now preserved after saving.
Improved Support for Retention Index Calibration
GC Image GCxGC-HRMS Edition 2.7 Release 1 includes improvements to support
generic retention index calibration.
- RI tables can be populated from areas and selected blobs in addition to included blobs and a blob set.
- An external RI table can be imported and appended to the existing table.
- The calibration function and R-squared value are shown in the title of RI Graph.
- RI tables have better error checking before applying changes.
If an error is found, the problem index row is automatically selected.
New Improvements for Mass Spectra Viewing and Centroiding
GC Image GCxGC-HRMS Edition 2.7 Release 2 has new improvements for
streamlining the work flow for mass calibration
- New option to open the chromatogram saved by
Export Recalibrated Centroid Image or Export Profile Data to Centroid.
- New OK and Export... button in Configure > Mass Calibration Model that
allows quick export of a recalibrated chromatogram.
In addition, the software has new improvements for the default MS mode including:
- The default MS mode can be set to either
Context, Point, Peak, or Blob/Area through Configure > Settings > Multi-Channel.
- MS Context mode now shows the Point spectrum in addition to Peak and Blob/Area spectra.
- MS Context mode now shows recalibrated spectra for all spectra.
- Improved default color scheme for spectra in the MS Viewer that make different types of spectra more distinct.
New Library Search Features
GC Image GCxGC-HRMS Edition 2.7 Release 2 includes significant improvements for Library Search features.
- Support In-source HiRes (Identity) search type for Batch search mode.
- Support NIST Library Search Presearch settings: Default, Fast, and Off
- New Multi-Spectral Filter settings for Search Library:
- Integrating spectra at % Apex: Integrate spectra with TIC values reaching at the specified percent of the apex of a blob or an area.
- Background Removal: Options for None, Start, and Start and End: Determines which portion of the blob to be used as a baseline for correcting library spectra.
- Ordinal Limit: Limit the search spectra to the largest number of intensities.
- Intensity Threshold: Limit the search spectra to intensities greater than the specified threshold.
- Range Limit: Limit the search spectra to the specified spectral range.
- Blob's current Hit List is loaded in MS Viewer when viewing a peak or blob spectrum.
- New Select column in the MS Viewer's Hit List table that clearly shows which hit is selected.
- Non-existent RI values in library search results are now displayed as empty instead of -1.
- "j3dcore-d3d" error when opening 3D View in 32-bit versions.
- Random socket access problem when importing Wiff files.
- Batch library search failed on very large spectra with unexpected error: -1073741819.
- Interactive library search retrieved RI without considering the "Use other non-polar column type..." option.
- Importing an old-version configuration did not set correct defaults for new library search settings.
- Wrong error message was shown when Configure > NIST Post Search constraints.
- MS Viewer's Spectrum List was not always visible after recalibrating a spectrum.
- MS Cube loaded and saved a SIC table with formulas incorrectly.
- Project > Vial > Delete Record threw an exception on an empty amount table.
What's New in Version 2.7 Release 1 (June 2017)
Large Data File Support
GC Image GCxGC-HRMS Edition 2.7 Release 1 includes significant improvements for reading and writing
large CDF files, which are used as the file store for MS data.
- The software now supports reading and writing of
larger CDF files that can contain more data than previously allowed.
The maximum data size is now only limited by the file
system and memory of the computer.
- Additional improvements yield faster read times for
CDF files on hard disk drives. GC Image now can open
large CDF files much faster!
Mass Spectra Centroiding, Calibration, and Viewing
GC Image GCxGC-HRMS Edition 2.7 Release 1 has new and improved operations for
mass calibration with HRMS data, including:
- The software supports exporting centroid data
extracted from profile data directly to a GCI file (with accompanying CDF
file), in addition to the option of exporting to a standalone CDF file.
- The software supports recalibrating centroid data
with or without profile data, in addition to recalibrating the profile data only.
After recalibration is configured, users can export
the recalibrated centroid data.
- The software has a new, default, context-based MS mode
that opens MS View for point, blob, or area spectra based on content.
If the mouse-click location is not within a blob or area, a point
spectrum is opened; otherwise, both the apex and summed spectra
for the blob or area are opened. If the location is within more
than one object, a popup menu allows selection.
MS Library Search for Area Objects
GC Image GCxGC-HRMS Edition 2.7 Release 1 introduces MS library search supports for areas.
- New Library Search actions directly search
for matches with spectra from all or selected areas.
- MS Viewer now can apply the hit list from a manual library search to
- Area properties now include a Hit List that allows
users to examine search results and select the preferred match.
- Library properties now can be added to the Areas
tab of the Blob Table.
New Quantifier Ion Properties
GC Image GCxGC-HRMS Edition 2.7 Release 1 has new properties and improved
operations for quantifier ions, including:
- A quantifier ion can be set as an internal standard for blobs and
areas for calibration and quantification, to support
isotope dilution analysis.
- New blob/area statistics for quantifier ions include retention
times, area, peak value, and volume.
New Calibration and Quantification Tools
GC Image GCxGC-HRMS Edition 2.7 Release 1 introduces new tools for calibration
and quantification. The process for generating calibration tables is
simplified by a new UI and options, including:
- New Quantification
menu options for tools to conveniently create calibration tables
directly from a set of chromatograms, and to load, edit, and save calibration tables.
- A new Calibration Table interface that allows users to examine
and edit individual calibrants. Calibration points and graphs of
the calibration curves are easily accessible through a tabbed view.
- New calibration function types, Quadratic and Piecewise Linear,
in addition to Linear.
- New calibration method files that allow calibration settings
to be customized and stored for reuse.
- Quantitation using ISTD Ions have been made easily available with the new
quantitation settings and UI improvements.
- Additional quantitation settings have been added to give users more
options. The quantification settings allow users to control the
targets to be quantified, how to measure the quantifier ions, the
uncalibrated response type, and if they want to allow extrapolation.
GC Image™ GCxGC-HRMS Edition Users' Guide © 2001–2017 by GC Image, LLC, and the
University of Nebraska.